Amphiphilic Effects of Chlorhexidine Digluconate on Rotational Mobility in Bacterial Outer Membranes and Liposomes
김태영, 차성권, 엄승일, 배문경, 배수경, 주지민, 윤일희, 김다솔, 장혜옥,
소속 상세정보
김태영 ( Kim Tae-Young ) - Pusan National University School of Dentistry Department of Dental Pharmacology
차성권 ( Cha Seong-Kweon ) - Pusan National University School of Dentistry Department of Dental Pharmacology
엄승일 ( Eom Seung-Il ) - Pusan National University School of Dentistry Department of Dental Pharmacology
배문경 ( Bae Moon-Kyoung ) - Pusan National University School of Dentistry Department of Oral Physiology
배수경 ( Bae Soo-Kyung ) - Pusan National University School of Dentistry Department of Dental Pharmacology
주지민 ( Ju Ji-Min ) - Pusan National University School of Dentistry Department of Dental Pharmacology
윤일희 ( Yun Il ) - Pusan National University School of Dentistry Department of Dental Pharmacology
김다솔 ( Kim Da-Sol ) - Pusan National University School of Dentistry Department of Dental Pharmacology
장혜옥 ( Jang Hye-Ock ) - Pusan National University School of Dentistry Department of Dental Pharmacology
Abstract
Chlorhexidine is a cationic bisbiguanide with broad antibacterial activity, and diverse activity encompassing gram-positive and gram-negative bacteria, yeasts, dermatophytes, and some lipophilic viruses. Chlorhexidine alters the permeability of the bacterial membrane. They also neutralize periodontal pathogens such as Streptococcus aureus, Porphyromonas gingivalis, and Prevotella intermedia. Porphyromonas gingivalis were cultured to isolate the Porphyromonas gingivalis outer membranes (OPG). Also, OPG were used to prepare large unilamellar liposomes with total lipids (OPGTL) extracted and prepared with the phospholipids mixture (PL). The effect of chlorhexidine digluconate on cell membrane kinetic changes was investigated using fluorescence polarization of n-(9-anthroyloxy) stearic acid with different rotational mobility depending on the probe substitution position (n) in the membrane phospholipid aliphatic chain. Chlorhexidine digluconate increased the hydrocarbon rotational mobility interior of the OPG, OPGTL and PL, but native and model membranes interfacial mobility were decreased. The sensitivity to increasing chlorhexidine digluconate effect on rotational mobility was proportional to the depth of probe position in descending order of 16-AP, 12-AS, 6-AS and 9-AS. Chlorhexidine digluconate disordering or ordering the effects on the membrane lipids may cause its bacteriostatic and bacteriocidal actions.
키워드
Bacterial outer membranes and liposomes; Chlorhexidine digluconate; Fluorescence probe technique; Hydrocarbon interior; Membrane interface
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